Mpb-64 – a Potential Marker for the Detection of Mycobacterium Tuberculosis Complex for the Detection of Tuberculosis
نویسندگان
چکیده
The diagnosis of extra-pulmonary tuberculosis (EPTB) remains a significant clinical difficulty, primarily because of the insufficient sensitivity of conventional bacteriologic methods for detecting Mycobacterium tuberculosis in extrapulmonary specimens. The PCR assays innovated in the developed countries are based on the genetic make-up of strains of Mycobacterial species circulating in those regions. Study also focuses on the distribution pattern of EPTB in different extra pulmonary specimens as well as in different age groups in males and females. For this study, 1200 extra pulmonary specimens for tuberculosis were collected from suspected patients from different hospitals of Delhi / NCR region. The specimens includes; menstrual blood, pus, urine, endometrial biopsy, endometrial pipelles, ascetic fluid, semen, pleural fluid, products of conception, endometrial tissue, endometrial curetting, body fluid, and tissue, cerebrospinal fluid. The DNA for PCR was extracted by the silica columns. Primers that amplify a 240 bp fragment of MPB64 from Mycobacterium tuberculosis complex were utilized. Out of 1200 specimens, females were more prevalent for the disease as 177 samples showed the positivity and 73 male samples were detected as positive for MTB. Considering age as a one of the parameters for our study, we found that positivity rate of TB detected in females in the age group of 41-60 was higher (i.e.10.6) and among the males TB was detected higher in the age group of 21-40 (i.e. 13.9). The positivity rate was detected higher in “Menstrual Blood” with the positivity rate of “28.7” and least in “Blood” with the positivity rate of “3.2”. These results demonstrate that PCR based on MPB64 gene target can be a highly specific and sensitive aid in the detection of M. tuberculosis from extra-pulmonary specimens.
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